IMMUNE PHENOTYPING OF FREE TUMOUR CELLS FOR EARLY DIAGNOSIS OF PERITONEAL CARCINOMATOSIS IN COLORECTAL CANCER
https://doi.org/10.33878/2073-7556-2019-18-1-39-45
Abstract
AIM: to test a method of free intraperitoneal colorectal cancer (CRC) cells isolation for the immunophenotyping and evaluation of mitomycin C hypertermic intraabdominal chemotherapy (HICT) PATIENTS AND METHODS: twenty-seven patients with CRC were included in the study.Peritoneal lavage prior and after HICT was performed for 10 of them. We defibrinated lavage fluid and stained concentrated tumour cells with monoclonal antibodies CD133 Vio Bright - FITC, CD24 PE, CD26 ECD, CD184 PC5 and CD44 PC7. FACS analysis was done after staining. RESULTS: patients with colon cancers had the increased expression of CD133 (p<0.001) and CD184 (p<0.05). Mesenteric lymph nodes involvement was followed by an increase of CD26 expression (p<0.05) in CD133+ cancer cells. The ratio of CD44/CD26 expression was increased in patients with peritoneal carcinomatosis (p<0.05). HICT lowers CD24 expression on CD133+ cancer stem cells. CONCLUSION: the method proposed for free peritoneal CRC cells identification and phenotyping can be used in clinical practice, particularly for evaluating the HICT efficacy. Suppressive effect of HICT on cancer stem cells is detected.
About the Authors
Yu. A. Shelygin
State Scientific Centre for Coloproctology
Russian Federation
Russian Federation
I. V. Obraztsov
State Scientific Centre for Coloproctology
Russian Federation
Russian Federation
M. A. Sukhina
State Scientific Centre for Coloproctology
Russian Federation
Russian Federation
S. I. Achkasov
State Scientific Centre for Coloproctology
Russian Federation
Russian Federation
V. N. Kashnikov
State Scientific Centre for Coloproctology
Russian Federation
Russian Federation
O. I. Sushkov
State Scientific Centre for Coloproctology
Russian Federation
Russian Federation
K. R. Sayfutdinova
State Scientific Centre for Coloproctology
Russian Federation
Russian Federation
References
1. Rathore, R. Colorectal Cancer. / R.Rathore //In: Ferri's Clinical Advisor. New York: Elsevier. - 2018 -p. 314-316.e1.
2. Klaver, C.E. Recommendations and consensus on the treatment of peritoneal metastases of colorectal origin: a systematic review of national and international guidelines. / C.E.Klaver, H.Groenen, D.G.Morton et al.// Colorectal Dis. - 2017 Mar. - №19 (3). - р. 224-236.
3. Gelli, M. Strategies to prevent peritoneal carcinomatosis arising from colorectal cancer. / M.Gelli, J.F.Huguenin, C.Cerebelli et al. // Future Oncol. - 2017 Apr. - №13 (10). - р. 907-918.
4. Sibio, S. Detection methods and clinical significance of free peritoneal tumor cells found during colorectal cancer surgery. / S.Sibio, C.Fiorani, C.Stolfi et al. // World J. Gastrointest Surg. - 2015 Sep. 27. - №7 (9). -р. 178-84.
5. Passot, G. Intra-operative peritoneal lavage for colorectal cancer. / G.Passot, K.Mohkam, E.Cotte et al. // World J. Gastroenterol. - 2014. - №20. -р. 1935-1939.
6. Tsouma, A. Circulating tumor cells in colorectal cancer: detection methods and clinical significance. / A.Tsouma, C.Aggeli, N.Pissimissis et al. // Anticancer Res. - 2008. - №28. - р. 3945-3960.
7. Szarynska, M. Cancer stem cells as targets for DC-based immunotherapy of colorectal cancer. / M.Szarynska, A.Olejniczak, J.Kobiela et al. // Sci. Rep. - 2018 Aug 13. - №8 (1). - р. 12042.
8. Rosiq, S. Colonic Stem Cells Expression of Lgr5 and CD133 Proteins as Predictive Markers in Colorectal Cancer among Egyptian Patients. / S.Rosiq, O.Hammam, A.Abdelalim et al. // Open Access Maced. J. Med. Sci. -2018 Jun 7. - №6 (6). - р. 968-974.
9. Xi, J. Suppression of GRP78 sensitizes human colorectal cancer cells to oxaliplatin by downregulation of CD24. / J.Xi, Y.Chen, S.Huang et al. // Oncol. Lett. - 2018 Jun. - №15 (6). - р. 9861-9867.
10. Wang, X. CD24 promoted cancer cell angiogenesis via Hsp90-mediated STAT3/VEGF signaling pathway in colorectal cancer. / X.Wang, Y.Zhang, Y.Zhao et al. // Oncotarget. - 2016 Aug 23. - №7 (34). - р. 5566355676.
11. Larrinaga, G. Dipeptidyl-peptidase IV activity is correlated with colorectal cancer prognosis. / G.Larrinaga, I.Perez, B.Sanz et al. // PLoS One. - 2015 Mar 19. - №10 (3). - e0119436.
12. Amara, S. Stromal cell derived factor-1 and CXCR4 expression in colorectal cancer promote liver metastasis. / S.Amara, I.Chaar, M.Khiari et al. //Cancer Biomark. - 2015. - №15 (6). - р. 869-79.
13. Jin, J. CXCR3 expression in colorectal cancer cells enhanced invasion through preventing CXCR4 internalization. / J.Jin, Z.Zhang, H.Wang et al.// Exp. Cell Res. - 2018 Aug 7. - pii: S0014-4827(18)30657-8.
14. Mohamed, S.Y. The Prognostic Value of Cancer Stem Cell Markers (Notchl, ALDH1, and CD44) in Primary Colorectal Carcinoma. / S.Y.Mohamed, R.M.Kaf M.M.Ahmed et al. // J. Gastrointest Cancer. - 2018 Aug 23. - doi: 10.1007/s12029-018-0156-6.
15. Jing, F. Colon cancer stem cell markers CD44 and CD133 in patients with colorectal cancer and synchronous hepatic metastases. / F.Jing, H.J.Kim, C.H.Kim et al.// Int. J. Oncol. - 2015 Apr. - №46 (4). -p. 1582-8.
16. Сушков, О.И. Перитонеальный карциноматоз при раке толстой кишки. подходы к лечению (обзор литературы). / О.И.Сушков, С.И.Ачкасов // Колопроктология. - 2016. - №4 (58). - с. 69-79.
17. Vernon, H.M. The conditions of action of «trypsin» on fibrin J Physiol. / H.M.Vernon // 1901. - Jun 14. -№26 (6). - р. 405-426.
18. Lam, C.S. Prognostic significance of CD26 in patients with colorectal cancer. / C.S.Lam, A.H.Cheung, S.K.Wong et al. // PLoS One. - 2014 May 28. - №9 (5). -e98582.
19. Pang, R. A subpopulation of CD26+ cancer stem cells with metastatic capacity in human colorectal cancer. / R.Pang, W.L.Law, A.C.Chu et al. // Cell Stem. Cell. - 2010 Jun 4. - №6 (6). - р. 603-15.
20. Yanm Y. Concise Review: Emerging Role of CD44 in Cancer Stem Cells. / Yan Y., Zuo X., Wei D. // A Promising Biomarker and Therapeutic Target Stem Cells Transl Med. - 2015 Sep. - №4 (9). - р. 1033-43.
Review
For citations:
Shelygin Yu.A., Obraztsov I.V., Sukhina M.A., Achkasov S.I., Kashnikov V.N., Sushkov O.I., Sayfutdinova K.R. IMMUNE PHENOTYPING OF FREE TUMOUR CELLS FOR EARLY DIAGNOSIS OF PERITONEAL CARCINOMATOSIS IN COLORECTAL CANCER. Koloproktologia. 2019;18(1(67)):39-45. (In Russ.) https://doi.org/10.33878/2073-7556-2019-18-1-39-45
Views:
663
Email this article 
