INCIDENCE OF TOXIN - PRODUCING STRAINS OF CLOSTRIDIUM SPP. AMONG MEDICAL STAFF IN COLOPROCTOLOGY DEPARTMENT

INTRODUCTION. The spread of microorganisms of the genus Clostridium in the population is 15 %. This phenomenon has not been studied in coloproctology department in Russia. AIM. To estimate the spread of toxin - producing strains of Clostridium spp. among the medical staff in coloproctology department. MATERIALS AND METHODS. There were analysed 39 of intraluminal faeces. The material was examined for the presence of glutamate dehydrogenase, toxins A and B of C.difficile using the immunochromatographic method. To detect microorganisms a matrix-activated laser desorption ionization time-of-flight mass spectrometry technique was used. RESULTS. Positive tests for toxins A and B of C.difficile were in 28 (71,8 %) of 39 samples. We have identified the culture of the genus Clostridium in 24 (61,5 %) of the 39 samples of intraluminal faeces. 17 (70,8 %) of these samples had positive tests for C.difficile toxins and 7 (29,2 %) was negative. In addition to C. difficile (3) others Clostridium were identified: C.perfringens (17), C.bifermentas (4), C.tertium (3), C.disporicum (2), C.sordellii (2). CONCLUSION. C.difficile was identified in 3 (7,7 %) cases. The spread of Clostridium microorganisms in medical stuff is almost 5 times higher than the average in the population. Professional activity is a risk factor for the spread of toxic strains of the genus Clostridium.

clostridium difficile infection, pseudomembranous colitis, antibiotic-associated diarrhea, clostridial infection, medical staff, toxin - producing strains

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