Mucosal microbiota of the diverticula in inflammatory complications of diverticular disease (a pilot study)
R. R. Shakirov,
Yu. S. Pankratova,
A. M. Senina,
E. A. Boulygina,
O. S. Karaseva,
T. V. Grigoryeva,
D. R. Yarullina,
O. Y. Karpukhin https://doi.org/10.33878/2073-7556-2024-23-1-108-116
Abstract
PATIENTS AND METHODS: in this pilot study using classical culture methods and analysis of 16S rDNA libraries sequenced on the Illumina MiSeq platform, we characterized the mucosa-associated microbiota of four diverticula in resected colon specimens of patients.
RESULTS: in all the samples the abundance of Enterobacteria and the shift towards the predominance of Bacteroides in the ratio of Prevotella-to-Bacteroides (P/B) was detected. In three samples, Firmicutes prevailed over Bacteroidetes. Also, in three samples the balance in the microbial landscape was strongly shifted towards one genus: Bacteroidetes, Parvimonas, Akkermansia, or Bifidobacterium.
CONCLUSION: microbiota inside inflamed diverticula revealed the specific shifts in the intestinal microbiome that may contribute to the progression of inflammation in the diverticulum up to its inflammatory destruction.
About the Authors
R. R. Shakirov
Kazan State Medical University; Republican Clinical Hospital
Russian Federation
Russian Federation
Butlerova st., 49, Kazan, 420012
Orenburg tract, 138, Kazan, 420064
Yu. S. Pankratova
Kazan State Medical University; Republican Clinical Hospital
Russian Federation
Russian Federation
Butlerova st., 49, Kazan, 420012
Orenburg tract, 138, Kazan, 420064
A. M. Senina
Kazan Federal University
Russian Federation
Russian Federation
Kremlevskaya st., 18, Kazan, 420008
E. A. Boulygina
Kazan Federal University
Russian Federation
Russian Federation
Kremlevskaya st., 18, Kazan, 420008
O. S. Karaseva
Kazan Federal University
Russian Federation
Russian Federation
Kremlevskaya st., 18, Kazan, 420008
T. V. Grigoryeva
Kazan Federal University
Russian Federation
Russian Federation
Kremlevskaya st., 18, Kazan, 420008
D. R. Yarullina
Kazan Federal University
Russian Federation
Russian Federation
Kremlevskaya st., 18, Kazan, 420008
O. Y. Karpukhin
Kazan State Medical University; Republican Clinical Hospital
Russian Federation
Russian Federation
Butlerova st., 49, Kazan, 420012
Orenburg tract, 138, Kazan, 420064
References
1. Chen Y, Zhou J, Wang L. Role and mechanism of gut microbiota in human disease. Front Cell Infect Microbiol. 2021;11:625913. doi: 10.3389/fcimb.2021.625913
2. Hasan N, Yang H. Factors affecting the composition of the gut microbiota, and its modulation. Peer J. 2019;7:e7502. doi: 10.7717/peerj.7502
3. Yang M, Hong G, Jin Y, et al. Mucosal-associated microbiota other than luminal microbiota has a close relationship with diarrheapredominant irritable bowel syndrome. Front Cell Infect Microbiol. 2020;10:515614. doi: 10.3389/fcimb.2020.515614
4. Ardatskaya M.D., Achkasov S.I., Veselov V.V., et al. Klinicheskie rekomendatsii. Divertikulyarnaya bolezn’. Koloproktologia. 2021;20(3):10–27. (In Russ.). doi: 10.33878/2073-7556-2021-203-10-27
5. Reitano E, Francone E, Bona E, et al. Gut microbiota association with diverticular disease pathogenesis and progression: a systematic review. Dig Dis Sci. 2022. doi: 10.1007/s10620-022-07600-x
6. Schieffer KM, Sabey K, Wright JR, et al. The microbial ecosystem distinguishes chronically diseased tissue from adjacent tissue in the sigmoid colon of chronic, recurrent diverticulitis patients. Sci Rep. 2017;7(1):8467. doi: 10.1038/s41598-017-06787-8
7. Piccioni A, Franza L, Brigida M, et al. Gut microbiota and acute diverticulitis: role of probiotics in management of this delicate pathophysiological balance. J Pers Med. 2021;11(4):298. doi: 10.3390/jpm11040298
8. Moniuszko A, Rydzewska G. The effect of cyclic rifaximin therapy on symptoms of diverticular disease from the perspective of the gastroenterology outpatient clinic: a “real-life” study. Prz Gastroenterol. 2017;12(2):145–51. doi: 10.5114/pg.2017.68167
9. Yarullina DR, Shafigullin MU, Sakulin KA, et al. Characterization of gut contractility and microbiota in patients with severe chronic constipation. PLoS ONE. 2020;15(7):e0235985. doi: 10.1371/journal.pone.0235985
10. Wedel T, Barrenschee M, Lange C, et al. Morphologic basis for developing diverticular disease, diverticulitis, and diverticular bleeding. Viszeralmedizin. 2015;31(2):76–82. doi: 10.1159/000381431
11. Sheth A, Floch M. Probiotics and diverticular disease. Nutr Clin Pract. 2009;24(1):41–4. doi: 10.1177/0884533608329230
12. Sekirov I, Russell SL, Antunes LC, et al. Gut microbiota in health and disease. Physiol Rev. 2010;90(3):859–904. doi: 10.1152/physrev.00045.2009
13. Di Pierro F. Gut microbiota parameters potentially useful in clinical perspective. Microorganisms. 2021;9(11):2402. doi: 10.3390/ microorganisms9112402
14. Ley RE, Turnbaugh PJ, Klein S, et al. Microbial ecology: human gut microbes associated with obesity. Nature. 2006;444(7122):1022–3. doi: 10.1038/4441022a
15. Pascale A, Marchesi N, Marelli C, et al. Microbiota and metabolic diseases. Endocrine. 2018;61(3):357–371. doi: 10.1007/s12020018-1605-5
16. Duan R, Zhu S, Wang B, et al. Alterations of gut microbiota in patients with irritable bowel syndrome based on 16s rrna-targeted sequencing: A systematic review. Clin Transl Gastroenterol. 2019;10(2):e00012. doi: 10.14309/ctg.0000000000000012
17. Linninge C, Roth B, Erlanson-Albertsson C, et al. Abundance of Enterobacteriaceae in the colon mucosa in diverticular disease. World J Gastrointest Pathophysiol. 2018;9(1):18–27. doi: 10.4291/wjgp. v9.i1.18
18. Ang MY, Dymock D, Tan JL, et al. Genome sequence of Parvimonas micra strain A293, isolated from an abdominal abscess from a patient in the United Kingdom. Genome Announc. 2013;1(6):e01025–13. doi: 10.1128/genomeA.01025-13
19. Wang Q, Huang S-Q, Li C-Q, et al. Akkermansia muciniphila may determine chondroitin sulfate ameliorating or aggravating osteoarthritis. Front Microbiol. 2017;8:1955. doi: 10.3389/ fmicb.2017.01955
20. Chen J, Chen X, Ho CL. Recent development of probiotic bifidobacteria for treating human diseases. Front Bioeng Biotechnol. 2021;9:770248. doi: 10.3389/fbioe.2021.770248
21. Gueimonde M, Ouwehand A, Huhtinen H, et al. Qualitative and quantitative analyses of the bifidobacterial microbiota in the colonic mucosa of patients with colorectal cancer, diverticulitis, and inflammatory bowel disease. World J Gastroenterol. 2007;13:3985–9. doi: 10.3748/wjg.v13.i29.3985
22. Kvasnovsky CL, Leong LEX, Choo JM, et al. Clinical and symptom scores are significantly correlated with fecal microbiota features in patients with symptomatic uncomplicated diverticular disease: A pilot study. Eur J Gastroenterol Hepatol. 2018;30:107–12. doi: 10.1097/MEG.0000000000000995
23. Barbara G, Scaioli E, Barbaro MR, et al. Gut microbiota, metabolome and immune signatures in patients with uncomplicated diverticular disease. Gut. 2017;66(7):1252–61. doi: 10.1136/gutjnl-2016-312377
24. Falagas ME, Siakavellas E. Bacteroides, Prevotella, and Porphyromonas species: a review of antibiotic resistance and therapeutic options. Int J Antimicrob Agents. 2000;15(1):1–9. doi: 10.1016/s0924-8579(99)00164-8
25. Arumugam M, Raes J, Pelletier E, et al. Enterotypes of the human gut microbiome. Nature. 2011;473(7346):174–80. doi: 10.1038/nature09944
26. Wu GD, Chen J, Hoffmann C, et al. Linking long-term dietary patterns with gut microbial enterotypes. Science. 2011;334(6052):105–8. doi: 10.1126/science.1208344
27. Koeth RA, Wang Z, Levison BS, et al. Intestinal microbiota metabolism of L-carnitine, a nutrient in red meat, promotes atherosclerosis. Nat Med. 2013;19(5):576–85. doi: 10.1038/nm.3145
28. Roager HM, Licht TR, Poulsen SK, et al. Microbial enterotypes, inferred by the Prevotella-to-Bacteroides ratio, remained stable during a 6-month randomized controlled diet intervention with the new nordic diet. Appl Environ Microbiol. 2014;80(3):1142–9. doi: 10.1128/AEM.03549-13
Review
For citations:
Shakirov R.R., Pankratova Yu.S., Senina A.M., Boulygina E.A., Karaseva O.S., Grigoryeva T.V., Yarullina D.R., Karpukhin O.Y. Mucosal microbiota of the diverticula in inflammatory complications of diverticular disease (a pilot study). Koloproktologia. 2024;23(1):108-116. https://doi.org/10.33878/2073-7556-2024-23-1-108-116
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