Mucosal microbiota of the diverticula in inflammatory complications of diverticular disease (a pilot study)
R. R. Shakirov,
Yu. S. Pankratova,
A. M. Senina,
E. A. Boulygina,
O. S. Karaseva,
T. V. Grigoryeva,
D. R. Yarullina,
O. Y. Karpukhin https://doi.org/10.33878/2073-7556-2024-23-1-108-116
Abstract
PATIENTS AND METHODS: in this pilot study using classical culture methods and analysis of 16S rDNA libraries sequenced on the Illumina MiSeq platform, we characterized the mucosa-associated microbiota of four diverticula in resected colon specimens of patients.
RESULTS: in all the samples the abundance of Enterobacteria and the shift towards the predominance of Bacteroides in the ratio of Prevotella-to-Bacteroides (P/B) was detected. In three samples, Firmicutes prevailed over Bacteroidetes. Also, in three samples the balance in the microbial landscape was strongly shifted towards one genus: Bacteroidetes, Parvimonas, Akkermansia, or Bifidobacterium.
CONCLUSION: microbiota inside inflamed diverticula revealed the specific shifts in the intestinal microbiome that may contribute to the progression of inflammation in the diverticulum up to its inflammatory destruction.
About the Authors
R. R. Shakirov
Kazan State Medical University; Republican Clinical Hospital
Russian Federation
Russian Federation
Butlerova st., 49, Kazan, 420012
Orenburg tract, 138, Kazan, 420064
Yu. S. Pankratova
Kazan State Medical University; Republican Clinical Hospital
Russian Federation
Russian Federation
Butlerova st., 49, Kazan, 420012
Orenburg tract, 138, Kazan, 420064
A. M. Senina
Kazan Federal University
Russian Federation
Russian Federation
Kremlevskaya st., 18, Kazan, 420008
E. A. Boulygina
Kazan Federal University
Russian Federation
Russian Federation
Kremlevskaya st., 18, Kazan, 420008
O. S. Karaseva
Kazan Federal University
Russian Federation
Russian Federation
Kremlevskaya st., 18, Kazan, 420008
T. V. Grigoryeva
Kazan Federal University
Russian Federation
Russian Federation
Kremlevskaya st., 18, Kazan, 420008
D. R. Yarullina
Kazan Federal University
Russian Federation
Russian Federation
Kremlevskaya st., 18, Kazan, 420008
O. Y. Karpukhin
Kazan State Medical University; Republican Clinical Hospital
Russian Federation
Russian Federation
Butlerova st., 49, Kazan, 420012
Orenburg tract, 138, Kazan, 420064
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For citations:
Shakirov R.R., Pankratova Yu.S., Senina A.M., Boulygina E.A., Karaseva O.S., Grigoryeva T.V., Yarullina D.R., Karpukhin O.Y. Mucosal microbiota of the diverticula in inflammatory complications of diverticular disease (a pilot study). Koloproktologia. 2024;23(1):108-116. https://doi.org/10.33878/2073-7556-2024-23-1-108-116
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