gnckКолопроктологияKoloproktologia2073-75562686-7303Russian Association of Coloproctology10.33878/2073-7556-2023-22-4-24-32gnck-1839Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL ARTICLESМониторинг метаболизма в стенке толстой кишки после повреждения грудного отдела спинного мозга (экспериментальное исследование)Metabolism in the large intestine wall after injury of the thoracic spinal cord (experimental study)https://orcid.org/0000-0001-6943-9757БалеевМ. С.BaleevMikhail S.

Балеев Михаил Сергеевич — к.м.н., врач-хирург,

пл. Минина и Пожарского, д. 10/1, г. Нижний Новгород, 603005

Mikhail S. Baleev,

10/1 Minin and Pozharsky Sq., Nizhny Novgorod, 603005

polikarpova-e@yandex.ruhttps://orcid.org/0000-0003-4769-417XКиселеваЕ. Б.KiselevaE. B.

Киселева Елена Борисовна — к.б.н., старший научный сотрудник,

пл. Минина и Пожарского, д. 10/1, г. Нижний Новгород, 603005

Elena B. Kiseleva,

10/1 Minin and Pozharsky Sq., Nizhny Novgorod, 603005

https://orcid.org/0000-0001-5368-8396БедеринаЕ. Л.BederinaE. L.

Бедерина Евгения Львовна — врач-патологоанатом,

пл. Минина и Пожарского, д. 10/1, г. Нижний Новгород, 603005

10/1 Minin and Pozharsky Sq., Nizhny Novgorod, 603005

https://orcid.org/0000-0002-5158-7089ЛогиноваМ. М.LoginovaM. M.

Логинова Мария Максимовна — младший научный сотрудник,

пл. Минина и Пожарского, д. 10/1, г. Нижний Новгород, 603005

10/1 Minin and Pozharsky Sq., Nizhny Novgorod, 603005

https://orcid.org/0000-0002-3207-7227ШирмановаМ. В.ShirmanovaM. V.

Ширманова Марина Вадимовна — к.б.н., заместитель директора по науке, заведующий научной лабораторией, лауреат премии президента в области науки и инноваций для молодых учёных, 

пл. Минина и Пожарского, д. 10/1, г. Нижний Новгород, 603005

Marina V. Shirmanova,

10/1 Minin and Pozharsky Sq., Nizhny Novgorod, 603005

ФраерманА. П.FraermanA. P.

Фраерман Александр Петрович — д.м.н., профессор, заслуженный деятель науки РСФСР, лауреатГосударственной премии России, консультант,

Московское ш., д. 144, г. Нижний Новгород, 603028

Alexander P. Fraerman,

144 Moscow highway, Nizhny Novgorod, 603028

https://orcid.org/0000-0003-3253-8211ЩеславскийВ. И.ScheslavskyV. I.

Щеславский Владислав Игоревич — доктор философии (PhD), заведующий научной лабораторией,

пл. Минина и Пожарского, д. 10/1, г. Нижний Новгород, 603005

Vladislav I. Scheslavsky,

10/1 Minin and Pozharsky Sq., Nizhny Novgorod, 603005

https://orcid.org/0000-0002-8386-7157ГладковаН. Д.GladkovaN. D.

Гладкова Наталья Дорофеевна — д.м.н., профессор, заведующий научной лабораторией, 

пл. Минина и Пожарского, д. 10/1, г. Нижний Новгород, 603005

Natalya D. Gladkova,

10/1 Minin and Pozharsky Sq., Nizhny Novgorod, 603005

https://orcid.org/0000-0002-9555-190XРябковМ. Г.RyabkovM. G.

Рябков Максим Георгиевич — д.м.н., доцент, ведущий научный сотрудник, 

пл. Минина и Пожарского, д. 10/1, г. Нижний Новгород, 603005

Maxim G. Ryabkov,

10/1 Minin and Pozharsky Sq., Nizhny Novgorod, 603005

НИИ экспериментальной онкологии и биомедицинских технологий. ФГБУ «Приволжский исследовательский медицинский университет» Минздрава РоссииРоссияInstitute of Experimental Oncology and Biomedical Technologies, Privolzhsky Research Medical UniversityRussian FederationГБУЗ Нижегородской области «ГКБ №39», Нижегородский городской нейрохирургический центр имени профессора А.П. ФраерманаРоссияGBUZ NO “City clinical hospital No. 39”, Nizhny Novgorod City Neurosurgical Center named after Professor A.P. FraermanRussian Federation2023211220232242432Copyright © Балеев М.С., Киселева Е.Б., Бедерина Е.Л., Логинова М.М., Ширманова М.В., Фраерман А.П., Щеславский В.И., Гладкова Н.Д., Рябков М.Г., 20232023Балеев М.С., Киселева Е.Б., Бедерина Е.Л., Логинова М.М., Ширманова М.В., Фраерман А.П., Щеславский В.И., Гладкова Н.Д., Рябков М.Г.Baleev M.S., Kiseleva E.B., Bederina E.L., Loginova M.M., Shirmanova M.V., Fraerman A.P., Scheslavsky V.I., Gladkova N.D., Ryabkov M.G.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.ruproctology.com/jour/article/view/1839Цель

Цель: изучить динамику метаболических процессов в тканях толстой кишки в остром периоде спинальной травмы.

Материалы и методы

Материалы и методы: эксперимент проводился на лабораторных животных — крысах линии Wistar (n = 20). Спинальную травму моделировали полным пересечением спинного мозга на уровне Th5-Th6 позвонков. Оценку метаболических изменений в кишечной стенке проводили до травмы, через 3 и 24 часа после пересечения спинного мозга. Метаболизм оценивали in vivo с помощью технологии флуоресцентного времяразрешенного макроимиджинга (макроFLIM) по автофлуоресценции в спектральном канале метаболического кофактора НАД(Ф)Н.

Результаты

Результаты: зафиксировано статистически значимое (p = 0,041) повышение среднего времени жизни флуоресценции (τm) на 12% и времени жизни длинной компоненты (τ2) кофактора НАД(Ф)Н на 13% (р = 0,008) через 24 часа после травмы. Наблюдаемые с помощью FLIM изменения указывают на рост интенсивности метаболизма в стенке толстой кишки в остром периоде спинальной травмы.

Заключение

Заключение: впервые в эксперименте in vivo показано, что острый период спинальной травмы сопровождается увеличением метаболической активности в тканях толстой кишки. Выявленные закономерности подтверждают значимость роли толстой кишки в компенсаторных реакциях на спинальную травму, открывают новые возможности терапии в остром периоде травмы спинного мозга.

Ailm

Ailm: to assess metabolic processes in the serous layer of the large intestine in the acute period of spinal injury.

Materials and Methods

Materials and Methods: the experiment was carried out on laboratory animals — Wistar rats (n = 20). Spinal injury was simulated by complete transection of the spinal cord at the level of Th5-Th6 vertebrae. Metabolic changes in the intestinal wall were assessed before injury, 3 and 24 hours after spinal cord transection. Metabolism was assessed in vivo using fluorescence time-resolved macroimaging technology (macroFLIM) by autofluorescence in the spectral channel of the metabolic cofactor NAD(P)H.

Results

Results: a significant (p = 0.041) increase in the mean fluorescence lifetime (τm) by 12% and the lifetime of the long component (τ2) of the NAD(P)H cofactor by 13% (p = 0.008) was detected 24 hours after injury. MacroFLIM showed an increase in the intensity of metabolism in the large intestine wall.

Conclusion

Conclusion: for the first time in an in vivo experiment, it has been shown that the acute period of spinal injury is accompanied by an increase in metabolic activity in the tissues of the large intestine. The revealed phenomenon confirms the role of the large intestine in compensatory reactions to spinal injury and open up new possibilities for therapy in the acute period of spinal cord injury.

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The authors declare that there are no conflicts of interest present.